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Smartphone based lateral flow immunoassay quantifications.

¹Department of Biomedical Engineering, Kyungil University, Gyeongsan 38428, Republic of Korea.

Journal of Immunological Methods

|August 22, 2024

Summary

Smartphones are increasingly used as readers for quantitative Lateral Flow Immunoassay (LFI) systems, enabling rapid, on-site diagnostics for various applications like disease detection and contamination testing.

Keywords:

Lateral flow immunoassay Point-of-care testing Quantitative analysis Smartphone

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Area of Science:

Biomedical Engineering
Analytical Chemistry
Point-of-Care Diagnostics

Background:

Lateral Flow Immunoassay (LFI) offers simple, disposable qualitative analysis.
Limitations in quantitative analysis of traditional LFI necessitate advanced reader systems.
The need for portable, user-friendly, and rapid diagnostic tools is growing.

Purpose of the Study:

To review research on utilizing smartphones as readers for quantitative LFI systems.
To explore the classification and performance enhancement of smartphone-based LFI systems.
To highlight the application of these systems in diverse fields, including disease diagnosis and contamination detection.

Main Methods:

Smartphones leverage high-performance cameras as sensors and powerful processors for signal conversion and analysis.
Systems are categorized based on the label particles employed in the assay.
Performance improvement strategies for different label particle types are examined.

Main Results:

Smartphones provide a mobile, user-friendly interface for quantitative LFI, integrating data acquisition, analysis, and transmission.
Successful applications demonstrated in food/environmental contamination, drug detection, and human/animal disease diagnostics.
Recent investigations include smartphone-based LFI for COVID-19 diagnosis.

Conclusions:

Smartphones are effective readers for quantitative LFI, enhancing accessibility and utility of diagnostic tests.
The versatility of smartphones supports a wide range of on-site diagnostic applications.
Continued development promises further improvements in the performance and scope of smartphone-based LFI systems.