[Enzyme engineering in microbial biosynthesis of terpenoids: progress and perspectives]

Area of Science:

• Biotechnology and Synthetic Biology
• Enzyme Engineering
• Metabolic Engineering

Background:

• Terpenoids are valuable compounds with diverse applications in food, cosmetics, and cleaning products.
• Microbial biosynthesis offers a sustainable route for terpenoid production.
• Natural enzymes in terpenoid synthesis exhibit limitations in activity, specificity, and stability, hindering efficiency.

Purpose of the Study:

• To review strategies for modifying key enzymes in microbial terpenoid biosynthesis.
• To highlight advancements in enzyme engineering for improved terpenoid production.
• To discuss future challenges and directions in this field.

Main Methods:

• Enzyme engineering approaches to enhance catalytic activity and stability.
• Strategies for altering enzyme specificity in terpenoid synthesis pathways.
• Multi-enzyme collaboration to promote mass transfer and pathway efficiency.

Main Results:

• Significant improvements in enzyme catalytic activity, specificity, and stability have been achieved.
• Engineered enzymes provide robust support for sustainable terpenoid production.
• Modified enzymes overcome natural limitations, increasing biosynthesis efficiency.

Conclusions:

• Enzyme engineering is crucial for optimizing microbial terpenoid biosynthesis.
• Continued research in enzyme engineering will drive sustainable production of valuable terpenoids.
• Addressing challenges in enzyme engineering will unlock further potential in the field.