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Updated: Jun 15, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Quantification of CH and NH/π-Stacking Interactions in Cells Using Nuclear Magnetic Resonance Spectroscopy

Xiaoxu Chen1,2,3, Xueying Zhang1,2,3,4, Jingfei Chen1,2,3

  • 1Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

Analytical Chemistry
|August 23, 2024
PubMed
Summary

Protein stacking interactions, like CH/π and NH/π, are weaker inside Escherichia coli cells than in solution. This weakening affects protein dynamics and conformational flexibility within the cellular environment.

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