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Deficiency of Trps1 in Cementoblasts Impairs Cementogenesis and Tooth Root Formation
Kaoru Fujikawa1,2, Mairobys Socorro1, Lyudmila Lukashova1
1Center for Craniofacial Regeneration, Department of Oral and Craniofacial Sciences, School of Dental Medicine, University of Pittsburgh, 501 Salk Pavilion, 335 Sutherland Drive, Pittsburgh, PA, 15213, USA.
Calcified Tissue International
|August 23, 2024
Summary
The Trps1 transcription factor is crucial for cementum formation, regulating cementoblast function and key protein expression. Its deficiency leads to shorter roots and impaired cementum development, highlighting its role in mineralized tissue regulation.
Area of Science:
- Oral Biology
- Mineralized Tissue Development
- Transcriptional Regulation
Background:
- Cementum formation mechanisms are poorly understood.
- The role of Trps1 transcription factor in cementogenesis is unknown.
Purpose of the Study:
- Investigate the transcriptional regulation of cementum formation.
- Determine the consequences of Trps1 deficiency in cementoblasts.
Main Methods:
- Utilized Trps1 conditional knockout (cKO) mice (Trps1Col1a1 cKO).
- Performed micro-computed tomography, histological, and immunohistochemical analyses.
- Quantified collagen fiber organization in the periodontal ligament.
Main Results:
- Trps1Col1a1 cKO mice exhibited shorter roots and thinner mineralized tissues.
- Reduced cellular cementum area and localized acellular cementum deficiencies were observed.
- Decreased Osterix and tissue-nonspecific alkaline phosphatase (TNAP) levels and disorganized periodontal collagen fibers were noted.
Conclusions:
- Identified Trps1 as a key regulator of cellular and acellular cementum formation.
- Trps1 supports cementoblast function by upregulating Osterix and TNAP expression.
- Trps1 deficiency impairs cementogenesis and periodontal structure.
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