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Comprehensive transcriptomic profiling of T-2 toxin-induced nephrotoxicity in mice
Guoquan Wu1, Xuan Wu2, Yige Wu1
1National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Ecotoxicology and Environmental Safety
|August 23, 2024
Summary
T-2 toxin exposure causes kidney damage in mice, affecting cellular processes and gene expression. This study reveals molecular mechanisms of T-2 toxin nephrotoxicity over time.
Area of Science:
- Environmental Toxicology
- Molecular Biology
- Renal Pathology
Background:
- T-2 toxin, a trichothecene mycotoxin, is a global environmental pollutant with known nephrotoxic effects.
- The precise molecular mechanisms underlying T-2 toxin-induced kidney damage remain incompletely understood.
Purpose of the Study:
- To investigate the molecular mechanisms of T-2 toxin-induced nephrotoxicity.
- To analyze gene expression changes in mouse kidneys at different time points post-exposure.
Main Methods:
- Mice were administered a single dose of T-2 toxin (2 mg/kg body weight).
- Kidney function and ultrastructure were assessed at 1, 3, and 7 days post-exposure.
- Transcriptomic analysis was performed on kidney tissues.
Main Results:
- Histopathology showed tubular degeneration, necrosis, and epithelial cell shedding.
- Electron microscopy revealed mitochondrial swelling and vacuolization.
- Transcriptomic analysis identified significant gene expression changes, including alterations in cell cycle, p53 signaling, cellular senescence, and ribosomal pathways.
Conclusions:
- This study provides the first comprehensive transcriptomic analysis of T-2 toxin-induced nephrotoxicity at multiple time points.
- The findings elucidate the molecular mechanisms of T-2 toxin nephrotoxicity at the mRNA expression level.
- Temporal gene expression patterns and metabolic disturbances during recovery were identified.
