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Trinitroglycerin-loaded chitosan nanogels accelerate angiogenesis in wound healing process
Khatereh Asadi1, Negar Azarpira2, Reza Heidari3
1Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Nanotechnology, School of Advanced Medical Science and Technology, Shiraz University of Medical Sciences, Shiraz, Iran; Guilan Road Trauma Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Trinitroglycerin-loaded chitosan nanogels accelerate wound healing by promoting collagen and angiogenesis while reducing inflammation. This topical therapy shows promise for enhanced wound repair and reduced scarring.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Trinitroglycerin (TNG) exhibits angiogenic, antibacterial, and antioxidative properties beneficial for wound healing.
- Clinical use of TNG is hindered by complications and a short nitric oxide (NO) half-life.
- Chitosan nanogels offer a potential delivery system to overcome TNG limitations.
Purpose of the Study:
- To develop and characterize Trinitroglycerin-loaded chitosan nanogels (TNG-Ngs).
- To evaluate the in-vitro and in-vivo efficacy of TNG-Ngs for wound healing applications.
- To assess the potential of TNG-Ngs as a topical therapy for skin wound repair.
Main Methods:
- Preparation and physiochemical characterization of TNG-Ngs.
- In-vitro biocompatibility and scratch assays using HUVECs.
- In-vivo assessment on a full-thickness skin wounds model.
- Histopathological and immunohistochemistry analyses (α-SMA, CD31).
Main Results:
- TNG-Ngs demonstrated favorable particle size and distribution, with a loading capacity of 70.2% and encapsulation efficiency of 2.1%.
- Enhanced HUVEC migration with no observed cytotoxicity in vitro.
- In-vivo studies showed complete wound closure, improved skin component formation, reduced scarring, enhanced granulation tissue, and increased collagen deposition.
- Significant upregulation of α-SMA and CD31 expression, indicating enhanced angiogenesis and tissue remodeling.
Conclusions:
- TNG-Ngs effectively accelerate wound healing by promoting collagen deposition and angiogenic activity.
- The nanogel formulation reduces inflammation and improves vascularization in the wound area.
- TNG-Ngs represent a promising and effective topical therapy for wound healing.
