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Updated: Jul 22, 2026
The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques
Published on: July 3, 2014
miRNA expression signatures induced by pasteurella multocida infection in goats lung
Feng Xu1, Hao Zheng2, Xia Dong2
1Animal Genetics and Breeding Key Laboratory of Sichuan Province, Animal Science Academy of Sichuan Province, Chengdu, China.
Abstract:
MicroRNAs (miRNAs) are important regulators of gene expression and are involved in bacterial pathogenesis and host-pathogen interactions. In this study, we investigated the function of miRNAs in the regulation of host responses to Pasteurella multocida infection. Using next-generation sequencing, we analyzed miRNA expression pattern and identified differentially expressed miRNAs in Pasteurella multocida-infected goat lungs. In addition, we investigated the function of differentially expressed miRNAs andtheir targeted signaling pathways in bacterial infection processes. The results showed that Pasteurella multocida infection led to 69 significantly differentially expressed miRNAs, including 28 known annotated miRNAs with miR-497-3p showing the most significant difference. Gene target prediction and functional enrichment analyses showed that the target genes were mainly involved in cell proliferation, regulation of the cellular metabolic process, positive regulation of cellular process, cellular senescence, PI3K-Akt signaling pathway, FoxO signaling pathway and infection-related pathways. In conclusion, these data provide a new perspective on the roles of miRNAs in Pasteurella multocida infection.
Insights
This study reveals how microRNAs (miRNAs) regulate host responses during Pasteurella multocida infections in goats. Key miRNAs and their pathways were identified, offering insights into bacterial pathogenesis.
Area of Science:
- Molecular Biology
- Genomics
- Immunology
Background:
- MicroRNAs (miRNAs) are crucial gene regulators implicated in host-pathogen interactions.
- Understanding miRNA roles in bacterial infections like Pasteurella multocida is vital for developing new therapeutic strategies.
Purpose of the Study:
- To investigate the function of miRNAs in regulating host responses to Pasteurella multocida infection in goats.
- To identify differentially expressed miRNAs and their targeted signaling pathways during infection.
Main Methods:
- Next-generation sequencing was employed to analyze miRNA expression patterns in infected goat lungs.
- Bioinformatic analyses, including gene target prediction and functional enrichment, were performed.
Main Results:
- Pasteurella multocida infection induced significant differential expression of 69 miRNAs, with miR-497-3p being notably altered.
- Target genes were primarily associated with cell proliferation, metabolic processes, cellular senescence, and key signaling pathways (PI3K-Akt, FoxO).
Conclusions:
- This research provides novel insights into the regulatory roles of miRNAs in the host's response to Pasteurella multocida infection.
- The identified miRNAs and pathways represent potential targets for future interventions against bacterial pathogenesis.
