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Updated: Jun 15, 2025

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
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Serine protease inhibitor E2 protects against cartilage tissue destruction and inflammation in osteoarthritis by

Linzhu Wang1, Shuangshuang Chen1, Huizhen Zhang1

  • 1Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), College of Pharmacy, Harbin Medical University, Harbin, PR China.

Rheumatology (Oxford, England)
|August 24, 2024
PubMed
Abstract

Insights

Serine peptidase inhibitor clade E member 2 (serpinE2) shows protective effects in osteoarthritis (OA) by reducing cartilage degeneration and inflammation. Upregulating serpinE2 may offer new therapeutic strategies for OA treatment.

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Osteoarthritis (OA) is a degenerative joint disease with no current disease-modifying treatments.
  • Cartilage degeneration and inflammation are key pathological features of OA.

Purpose of the Study:

  • To identify pathogenic genes involved in OA.
  • To elucidate the underlying molecular mechanisms of these genes in OA pathogenesis.

Main Methods:

  • Combined single-cell and bulk transcriptome analysis of OA cartilage.
  • In vivo studies using a rat model of OA (monosodium iodoacetate-induced).
  • In vitro experiments on chondrocytes involving gene silencing and exogenous protein administration.

Main Results:

  • Serine peptidase inhibitor clade E member 2 (serpinE2) expression is elevated and hypomethylated in OA, correlating with collagen pathway activity.
  • Silencing serpinE2 worsened OA in rats, while exogenous serpinE2 ameliorated cartilage degeneration, pain, and synovitis.
  • Exogenous serpinE2 inhibited NLRP3 inflammasome activation, reduced reactive oxygen species (ROS), and suppressed NF-κB signaling in chondrocytes.

Conclusions:

  • SerpinE2 demonstrates a protective role in both cartilage and synovium in OA.
  • SerpinE2 gene therapy or agents that boost serpinE2 levels are potential therapeutic avenues for OA.
Keywords:
NF-κB signalling pathwayOAinflammationreactive oxygen speciesserpinE2

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