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Related Concept Videos

Aquaporins01:25

Aquaporins

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Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
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Regulation of Angiogenesis and Blood Supply01:24

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Replicative Cell Senescence02:15

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
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Related Experiment Video

Updated: Jun 15, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

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AQP1 differentially orchestrates endothelial cell senescence.

Khatereh Shabanian1, Taraneh Shabanian1, Gergely Karsai2

  • 1Center for Translational and Experimental Cardiology, Department of Cardiology, University Hospital Zurich, University of Zurich, 8952, Schlieren, Switzerland; University Heart Center, Department of Cardiology, University Hospital Zurich, Zurich, Switzerland.

Redox Biology
|August 24, 2024
PubMed
Summary

Aquaporin 1 (AQP1) drives endothelial cell senescence and impairs blood vessel formation by transporting hydrogen peroxide. Blocking AQP1 in aging cells can reverse senescence and restore angiogenic function, offering a therapeutic target for cardiovascular diseases.

Keywords:
AgingAngiogenesisAquaporin 1Endothelial senescenceEpigenetic modificationHydrogen peroxide

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Techniques to Induce and Quantify Cellular Senescence
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Area of Science:

  • Cardiovascular Biology
  • Cellular Senescence
  • Molecular Mechanisms

Background:

  • Aging-associated endothelial cell (EC) senescence drives cardiovascular diseases.
  • Mechanisms regulating EC senescence, particularly in response to oxidative stress, remain poorly understood.

Purpose of the Study:

  • To investigate the role of aquaporin 1 (AQP1) in hydrogen peroxide (H2O2)-induced endothelial cell senescence.
  • To elucidate the signaling pathways regulated by AQP1 in ECs and their impact on angiogenesis.

Main Methods:

  • Investigated AQP1 function in aortic ECs under normal and H2O2-induced senescence conditions.
  • Utilized AQP1 knockdown and selective blockade in senescent ECs.
  • Analyzed cell-cycle arrest, senescence-associated secretory phenotype (SASP), DNA damage, mitochondrial function, and angiogenesis.
  • Examined the role of AQP1 in CaMKII-AMPK, HDAC4, Mef2A, and eNOS signaling pathways.

Main Results:

  • AQP1 facilitates H2O2 transport, promoting EC senescence and impairing angiogenesis.
  • Disruption of AQP1 in proliferating ECs leads to senescence and reduced angiogenic capacity.
  • AQP1 blockade in senescent ECs rescues senescence phenotype and restores angiogenic competence.
  • AQP1 regulates EC senescence via the HDAC4-Mef2A-eNOS signaling axis.

Conclusions:

  • AQP1 is a critical regulator of EC senescence and angiogenic potential.
  • AQP1's role in H2O2 transport and downstream signaling makes it a potential therapeutic target for age-related cardiovascular diseases.