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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Updated: Jun 15, 2025

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

Recent progress on media for biological sample preparation.

Huasheng Lai¹, Gongke Li²

¹Jiangxi Province Key Laboratory of Pharmacology of Traditional Chinese Medicine, School of Pharmacy, Gannan Medical University, Ganzhou, 341000, China; School of chemistry, Sun Yat-Sen University, Guangzhou, 510006, China.

Journal of Chromatography. A

|August 24, 2024

Summary

This summary is machine-generated.

This review highlights advanced media for biological sample preparation, crucial for accurate analysis in diagnostics and forensics. It covers novel materials and their applications in analyzing various biological samples.

Keywords:

Biological samples Extraction Media Sample preparation

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Published on: March 19, 2019

Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Biological sample analysis is vital for disease diagnosis, forensics, and public safety.
Matrix interference from biological samples significantly hinders the accurate detection of trace analytes.
Effective sample preparation is essential for overcoming these limitations.

Purpose of the Study:

To review high-performance media for biological sample preparation and analysis over the past five years.
To explore the applications of these media across diverse biological sample types.
To provide conclusions and future outlooks on advancements in biological sample preparation media.

Main Methods:

Review of recent scientific literature (last 5 years) focusing on media for biological sample preparation.
Categorization and discussion of high-performance media including porous organic frameworks, imprinted polymers, hydrogels, ionic liquids, and bioactive media.
Analysis of media applications for liquid (body fluids), solid (hair, feces, tissues), and gas (exhale breath) samples.

Main Results:

Identification of several high-performance media categories demonstrating significant potential in biological sample preparation.
Demonstration of the versatility of these media in handling various biological matrices and analytes.
Overview of the current state and recent advancements in the field of biological sample preparation media.

Conclusions:

Advanced media significantly enhance the extraction, separation, enrichment, and purification of trace analytes from complex biological samples.
The reviewed media offer promising solutions for improving the accuracy and efficiency of biological sample analysis.
Continued development of novel media is crucial for addressing future challenges in biological sample preparation and analysis.