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Exploiting UPO versatility to transform rutin in more soluble and bioactive products
Abel Muñiz-Mouro1, Beatriz Gullón2, Gemma Eibes1
1CRETUS, Department of Chemical Engineering, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Galicia, Spain.
New Biotechnology
|August 24, 2024
Summary
Unspecific peroxygenases (UPOs) modified the natural antioxidant rutin, creating more soluble and bioactive products. Enzyme reusability was explored, facing stability challenges during recovery.
Area of Science:
- Biocatalysis
- Enzymology
- Natural Product Modification
Background:
- Unspecific peroxygenases (UPOs) offer versatile enzyme-based oxyfunctionalization.
- UPOs can modify natural antioxidants, producing high-value products.
- Rutin, a natural flavonoid antioxidant, is a potential substrate for UPO modification.
Purpose of the Study:
- To investigate the peroxygenation and peroxidation of rutin by Agrocybe aegerita UPO.
- To optimize UPO-based rutin modification for enhanced solubility and bioactivity.
- To evaluate the reusability and stability of UPO in an enzymatic membrane reactor.
Main Methods:
- Enzymatic modification of rutin using UPO from Agrocybe aegerita.
- Multivariable optimization of reaction conditions.
- Enzymatic membrane reactor for enzyme recovery and reusability assessment.
- Investigation of ascorbic acid's influence on product profile.
Main Results:
- UPO successfully modified rutin through peroxygenation and/or peroxidation followed by oligomerization.
- Optimization aimed to maximize soluble antioxidant production while preserving enzyme activity.
- Enzyme stability issues were identified during filtration stages in the membrane reactor.
- Ascorbic acid promoted the formation of hydroxylated rutin derivatives, increasing solubility and bioactivity.
Conclusions:
- UPO-mediated modification of rutin yields more soluble and potentially more bioactive compounds.
- Enzyme reusability presents challenges related to enzyme stability during recovery processes.
- Further research is needed to overcome enzyme stability limitations for industrial applications.
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