Related Experiment Video
Updated: May 2, 2026
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
Published on: April 16, 2016
A novel electrochemiluminescence aptasensor for ultrasensitive lincomycin detection using Ti3C2-TiO2-Ru probe
Huixin Zhang1, Zhihao Wei2, Feifei Zhang2
1College of Chemistry and Chemical Engineering, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao 266071, China; School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266071, China.
A novel electrochemiluminescence aptasensor using a self-enhancing Ti3C2-TiO2-Ru probe was developed for lincomycin (LIN) detection. This sensitive method accurately detects LIN residues in food, ensuring consumer safety.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Lincomycin (LIN) residues in food present significant human health risks.
- Highly sensitive and specific detection methods for LIN are crucial for food safety.
Purpose of the Study:
- To develop a novel electrochemiluminescence aptasensor for sensitive and specific detection of lincomycin.
- To utilize a self-enhancing Ti3C2-TiO2-Ru probe for improved detection performance.
Main Methods:
- Synthesis of Ti3C2-TiO2 composite via in situ method, utilizing Ti3C2 reducibility and TiO2 as a co-reaction accelerator.
- Immobilization of Ru(bpy)32+ onto the negatively charged Ti3C2-TiO2 carrier via electrostatic adsorption to form the self-enhancing probe.
- Development of an electrochemiluminescence aptasensor leveraging aptamer-LIN affinity and Ti-phosphate chelation for precise LIN detection.
Main Results:
- The developed aptasensor exhibited a low detection limit of 0.025 ng mL−1 and a wide detection range (1.0 × 10−1–1.0 × 104 ng mL−1).
- Accurate LIN detection in milk samples was achieved with high recovery rates (94.4%–106.0%).
Conclusions:
- The self-enhancing Ti3C2-TiO2-Ru probe-based electrochemiluminescence aptasensor offers excellent performance for LIN detection.
- This method provides a sensitive, specific, and reliable approach for monitoring lincomycin residues in food products.
