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Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

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Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
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Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

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The intestinal stem cell as a target: A review.

Nisreen Lutfi Faizo1

  • 1Department of Clinical Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

Medicine
|August 26, 2024
PubMed
Summary

Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is crucial for intestinal stem cell renewal. Dysregulation of LGR5, Wnt, and Notch signaling contributes to colorectal cancer (CRC) development and offers therapeutic targets.

Area of Science:

  • Gastroenterology
  • Oncology
  • Cell Biology

Background:

  • The human intestinal epithelium comprises diverse cells crucial for absorption, defense, and regeneration.
  • Intestinal stem cells (ISCs), located in crypts, maintain intestinal homeostasis and renewal.
  • Disruptions in ISC function and signaling pathways like Wnt and Notch are linked to colorectal cancer (CRC) development.

Purpose of the Study:

  • To review the intricate relationship between LGR5, Wnt signaling, Notch signaling, and colorectal cancer (CRC) pathogenesis.
  • To explore the role of LGR5+ cells as intestinal stem cells (ISCs) in maintaining intestinal homeostasis.
  • To discuss emerging therapeutic strategies targeting LGR5, cancer stem cells (CSCs), and key signaling pathways in CRC.

Main Methods:

  • Literature review synthesizing current research on LGR5, Wnt, and Notch signaling in intestinal homeostasis and CRC.

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Last Updated: Jun 15, 2025

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  • Analysis of studies investigating the expression and function of LGR5 in both normal intestinal stem cells and colorectal cancer.
  • Examination of therapeutic approaches targeting LGR5, CSCs, and related signaling pathways.

    • Main Results:

    • LGR5 expression is upregulated in colorectal cancer (CRC) and is a marker for intestinal stem cells (ISCs).
    • Wnt and Notch signaling pathways are critical for ISC self-renewal and homeostasis; their dysregulation promotes CRC.
    • LGR5 plays a regulatory role in Wnt signaling, linking ISC function to cancer development.

    Conclusions:

    • LGR5, Wnt, and Notch signaling pathways are fundamentally involved in the development of colorectal cancer (CRC).
    • Targeting LGR5, CSCs, and these signaling pathways represents a promising avenue for novel CRC therapies.
    • Understanding ISC regulation is key to developing effective strategies against colorectal cancer.