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Identification and validation of basement membrane-associated gene AGRN as prognostic and immune-associated biomarkers in colorectal cancer patients

  • 0Department of General Surgery Sciences, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, China.
Journal of Cellular and Molecular Medicine +

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August 26, 2024

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August 26, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Basement membranes (BMs) play a crucial role in colorectal cancer (COCA) progression. Our research identifies a 12-BM signature and AGRN as a key biomarker, improving patient survival prediction and offering new therapeutic insights.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genomics

Background

  • Colorectal cancer (COCA) presents a significant health challenge with a poor prognosis.
  • Emerging evidence highlights the involvement of basement membranes (BMs) in cancer progression.
  • Understanding the specific role of BMs in COCA is critical for improving patient outcomes.

Purpose Of The Study

  • To investigate the prognostic significance of basement membranes (BMs) in colorectal cancer (COCA).
  • To identify BMs-related genes and construct a predictive model for COCA patient survival.
  • To explore the relationship between BMs, immune infiltration, and drug sensitivity in COCA.

Main Methods

  • Utilized TCGA and GTEx databases for gene expression and patient data.
  • Employed Cox and LASSO regression for prognostic gene selection and risk model development.
  • Performed Kaplan-Meier analysis, drug sensitivity analysis, and in vitro experiments.

Main Results

  • A 12-BM-based signature was identified as an independent prognostic factor in COCA.
  • BMs were found to be enriched in cancer-associated pathways, correlating with immune cell infiltration and checkpoint activation.
  • Elevated AGRN levels were significantly associated with decreased progression-free survival and promoted tumour metastasis.

Conclusions

  • The 12-BM signature serves as a reliable prognostic tool for COCA patients.
  • AGRN is a dependable biomarker for predicting patient survival and prognosis in colorectal cancer.
  • Targeting BMs and AGRN may offer novel therapeutic strategies for COCA.

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