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In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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Identification of In Vivo Internalizing Cardiac-Specific RNA Aptamers.

Chandan Narayan1, Li-Hsien Lin1, Maya N Barros1

  • 1Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.

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|August 26, 2024
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Researchers developed novel aptamers for targeted cardiac drug delivery. These aptamers selectively bind to and enter heart cells, showing potential for treating heart disease and improving cardiac imaging.

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SELEXaptamercardiomyocyte targeting ligands

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Targeted therapeutic delivery to specific tissues is crucial for treating diseases, especially cardiac conditions.
  • Developing cardiac-specific ligands is essential for effective heart-targeted therapies.

Purpose of the Study:

  • To develop and evaluate novel aptamers with high specificity for ventricular cardiomyocytes.
  • To assess the potential of these aptamers for targeted drug delivery and cardiac imaging.

Main Methods:

  • Utilized both ex vivo and in vivo SELEX (Systematic Evolution of Ligands by Exponential Enrichment) to enrich aptamers.
  • Screened a library of 2'-fluoro modified aptamers for ventricular cardiomyocyte specificity.
  • Evaluated binding and internalization of lead aptamers in ex vivo and in vivo mouse models.

Main Results:

  • SELEX generated aptamers with significant cardiac specificity over non-cardiac tissues like liver and skeletal muscle.
  • Lead aptamer CA1 demonstrated selective in vivo targeting and delivery of a fluorophore to murine ventricular cardiomyocytes.
  • CA1 predominantly internalizes into cardiomyocytes, while CA41 primarily binds to the cell surface.

Conclusions:

  • Aptamers CA1 and CA41 show promise for targeted drug delivery in cardiac diseases.
  • These aptamers are suitable candidates for cardiac imaging applications.
  • The findings support the potential of aptamer technology in cardiovascular medicine.