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Updated: Jun 20, 2026
10:58
A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals
Published on: February 14, 2014
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Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals
Simon Haziza1,2, Radosław Chrapkiewicz1,2, Yanping Zhang1,2,3
1James H. Clark Center, Stanford University, Stanford, CA 94305, USA.
Biorxiv : the Preprint Server for Biology
|August 26, 2024
Summary
New TEMPO voltage-sensing technologies achieve high sensitivity for tracking neural oscillations up to 100 Hz. These tools enable detailed studies of neural dynamics and interactions in both healthy and diseased brains.
Area of Science:
- Neuroscience
- Biotechnology
- Optical Imaging
Background:
- Genetically encoded voltage indicators are crucial for monitoring neural activity.
- Existing voltage-imaging instrumentation lacks sensitivity for high-frequency neural oscillations.
- Tracking neural oscillations is vital for understanding brain function.
Purpose of the Study:
- To introduce two novel TEMPO voltage-sensing technologies for sensitive neural oscillation detection.
- To enable high-frequency voltage imaging in targeted cell populations.
- To investigate neural dynamics and cell-type interactions in vivo.
Main Methods:
- Development and application of Fiber-optic TEMPO for sensitive photometry.
- Utilizing the TEMPO mesoscope for wide-field voltage imaging.
- Recording neural oscillations up to ~100 Hz in freely moving and head-fixed mice.
Main Results:
- Fiber-optic TEMPO demonstrated ~10-fold increased sensitivity, enabling hour-long recordings.
- Uncovered cross-frequency coupling of theta and gamma oscillations during hippocampal ripples.
- TEMPO mesoscope revealed sensory-evoked excitatory-inhibitory interactions and traveling waves in the visual cortex.
Conclusions:
- TEMPO technologies significantly advance the capability to image neural voltage oscillations.
- These tools provide new insights into neural dynamics, including previously unreported wave propagation directions.
- Widespread applications are anticipated for probing brain oscillations in various neurological conditions.
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Overview
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