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Related Concept Videos

Patch Clamp01:18

Patch Clamp

5.4K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
5.4K

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Related Experiment Video

Updated: Jun 15, 2025

A Computer-assisted Multi-electrode Patch-clamp System
11:01

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

13.8K

Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.

Mighten C Yip1, Mercedes M Gonzalez1, Colby F Lewallen2

  • 1George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.

Biorxiv : the Preprint Server for Biology
|August 26, 2024
PubMed
Summary
This summary is machine-generated.

Patch-walking streamlines synaptic connection measurement in brain tissue. This efficient method significantly increases the number of probed neuronal connections compared to traditional techniques.

Keywords:
automationpaired recordingpatch clampsynaptic connectivity

More Related Videos

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

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Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology
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Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Published on: April 23, 2019

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Related Experiment Videos

Last Updated: Jun 15, 2025

A Computer-assisted Multi-electrode Patch-clamp System
11:01

A Computer-assisted Multi-electrode Patch-clamp System

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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

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Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology
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Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Published on: April 23, 2019

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Area of Science:

  • Neuroscience
  • Electrophysiology

Background:

  • Measuring synaptic connections in living brain tissue presents technical challenges.
  • The traditional patch clamping technique is labor-intensive and time-consuming for probing synaptic connections.

Purpose of the Study:

  • To develop a more efficient method for probing synaptic connections between neurons.
  • To improve the efficiency of patch clamping for synaptic analysis.

Main Methods:

  • Introduced a novel technique termed "patch-walking."
  • Involves reusing a cleaned patch clamp pipette for subsequent recordings while maintaining others.
  • This allows for "walking" across tissue to probe multiple connections sequentially.

Main Results:

  • Performed 136 patch clamp attempts with two pipettes, achieving a 52.2% success rate (71 recordings).
  • Probed 29 neuron pairs (58 bidirectional connections) at an average intersomatic distance of 91 μm, identifying 3 connections.
  • Patch-walking yielded 80-92% more probed connections in experiments with 10-100 cells compared to traditional methods.

Conclusions:

  • Patch-walking significantly enhances the efficiency of probing synaptic connections.
  • This method offers a substantial improvement over traditional patch clamping for synaptic analysis in neuroscience research.