Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Search research articles

Home
Research Domains
Chemical Sciences
Medicinal And Biomolecular Chemistry
Characterisation Of Biological Macromolecules
Integrating Network Pharmacology, Molecular Docking And Experimental Verification To Reveal The Mechanism Of Artesunate In Inhibiting Choroidal Melanoma.

Home
Research Domains
Chemical Sciences
Medicinal And Biomolecular Chemistry
Characterisation Of Biological Macromolecules
Integrating Network Pharmacology, Molecular Docking And Experimental Verification To Reveal The Mechanism Of Artesunate In Inhibiting Choroidal Melanoma.

Related Experiment Video

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Published on: April 7, 2023

Integrating network pharmacology, molecular docking and experimental verification to reveal the mechanism of artesunate in inhibiting choroidal melanoma.

Qing-Yue Ma¹, Yi-Chong Liu¹, Qian Zhang¹

¹Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, China.

Frontiers in Pharmacology

|August 26, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Keywords:

anti-cancer mechanism artesunate choroidal melanoma molecular docking

More Related Videos

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

Related Experiment Videos

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Published on: April 7, 2023

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

Artesunate (ART) shows potential against choroidal melanoma (CM) by inducing apoptosis and cell cycle arrest. This study validates ART's anti-cancer effects on CM through various assays, suggesting its therapeutic promise.

Area of Science:

Oncology
Pharmacology
Molecular Biology

Background:

Choroidal melanoma (CM) is a significant ocular malignancy with limited treatment options.
Artesunate (ART), derived from Artemisia annua, exhibits potential anti-tumor properties, but its mechanism in CM is not fully understood.

Purpose of the Study:

To investigate the anti-CM effects and underlying mechanisms of Artesunate (ART).
To explore ART's therapeutic potential for choroidal melanoma using integrated computational and experimental approaches.

Main Methods:

Network pharmacology identified ART targets and CM-related genes.
Molecular docking predicted ART-target binding affinity.
In vitro and in vivo assays validated ART's effects on CM cell proliferation, apoptosis, cell cycle, and ROS levels.

network pharmacology

Main Results:

ART demonstrated significant inhibition of CM cell proliferation and migration.
ART promoted apoptosis via p53 pathway activation and induced G0/G1 cell cycle arrest by inhibiting PI3K/AKT/mTOR.
ART increased intracellular ROS by activating the NRF2/HO-1 pathway, with in vivo studies confirming anti-tumor effects.

Conclusions:

Artesunate exhibits significant anti-cancer effects on choroidal melanoma.
ART's mechanisms involve apoptosis promotion, cell cycle arrest, and ROS elevation, highlighting its therapeutic potential for CM.