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Related Experiment Video

Updated: May 2, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
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Development of an Elastin-like Polypeptide-Based Nucleic Acid Delivery System Targeted to EGFR+ Bladder Cancer Cells

Aayush Aayush1, Saloni Darji1, Kiera M Estes1

  • 1Department of Chemistry & Purdue Institute for Cancer Research, Purdue University, Bindley Bioscience Center, West Lafayette, Indiana 47907, United States.

Biomacromolecules
|August 26, 2024
PubMed
Summary

This study introduces a novel elastin-like polypeptide (ELP) nanocarrier system for efficient nucleic acid (NA) delivery. These adaptable nanocarriers target bladder tumors effectively, offering a promising alternative to existing NA delivery methods.

Keywords:
bladder cancer therapeuticselastin-like polypeptideslayer-by-layer formulationnon-viral nucleic acid deliverynucleic acid encapsulationtargeted nanoparticles

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Genetic Engineering

Background:

  • Nucleic acid (NA)-based therapies offer precise genetic control but face delivery challenges.
  • Existing delivery systems like lipid nanoparticles and viral vectors have limitations in efficiency, stability, and immunogenicity.

Purpose of the Study:

  • To develop a versatile elastin-like polypeptide (ELP) carrier system for effective NA delivery.
  • To create adaptable nanocarriers (LENNs) for targeting challenging biological sites like bladder tumors.

Main Methods:

  • Utilized a layer-by-layer (LbL) formulation approach to create ELP-based nucleic acid nanoparticles (LENNs).
  • Physically characterized LENNs and evaluated their biological performance in murine and human bladder tumor cell lines.
  • Assessed binding, internalization, and cargo release kinetics within bladder tumor cells.

Main Results:

  • LENNs demonstrated efficient binding and rapid internalization into bladder tumor cells within 30-60 minutes.
  • The ELP carrier system successfully delivered NA cargos (siRNA to plasmids) to target cells.
  • The system showed flexibility in targeting ability, cargo size, and disassembly kinetics.

Conclusions:

  • Developed an agile, biomanufacturable, and tunable nanocarrier for NA delivery.
  • LENNs offer a promising alternative to current NA delivery methods, overcoming limitations of lipid nanoparticles and viral vectors.
  • This adaptable system holds potential for various therapeutic applications requiring targeted genetic material delivery.