Determining Ion-Pair Binding Affinities of Heteroditopic Receptor Systems
1Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Abstract:
Determining ion-pair affinities in heteroditopic receptor systems presents a persistent and significant challenge. The plethora of technical and experimental problems implicated in measuring ion-pair affinities have encouraged the use of several expedient experimental practices as a means of characterising ion-pair recognition behaviour. Exploiting a model heteroditopic receptor system, we interrogate the reliability of these methods and demonstrate that these commonly used techniques can be highly questionable and without extreme care can lead to incorrect conclusions.
Related Concept Videos
The Two-State Receptor Model
The binding affinity of a drug determines its interaction with...
Drug-Receptor Bonds
In...
Ligand Binding Sites
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding and Linkage
Cooperative Allosteric Transitions
Drug-Receptor Interactions
Several parameters, such as the drug's affinity for its receptor and its efficacy, which is its ability to activate the receptor, determine the drug's effect on the tissue....


