Antibacterial effect of Softsoap used to disinfect dentures

To evaluate the in vitro antibacterial effect of Softsoap and Efferdent used as solutions to disinfect Lucitone 199 poly(methyl methacrylate) (PMMA) resin used for dentures.

S. mutans and plaque bacteria were grown for 24 hours, and suspended to a concentration of 1x10⁶ cells/ml. Bacterial suspensions (0.2 mL) were added to the decontaminated PMMA discs placed in a 48-well culture plate and incubated for 3 days at 37°C. The discs were rinsed to remove the unbound bacterial cells and then incubated for 60 minutes with 5% and 1% dilutions (triplicates) of each of the detergent solutions (0.3 ml). Discs were rinsed and then MTT reagent (0.2 ml) was added and incubated for 2 hours, then overnight with a solubilizing agent. An aliquot from each well (0.1 ml) was transferred to a 96-well flat bottom plate and absorbance was measured to OD @ 595 nm (MTT) of four samples for each data point. Normalized data was compared and statistically analyzed using a three-way ANOVA with Student-Newman-Keuls on Rank data with P< 0.05 for significance. Additionally, data were double-checked with the Holm-Sidak test.

There was no statistically significant difference between testing media for C. albicans and mixed plaque (P= 0.078) or testing duration in time at 24 hours and 21 days (P= 0.07). Statistically significant differences were found between all treatment solutions group combinations (P< 0.001) except for 30% Softsoap versus Efferdent (P= 0.056).

There was no statistically significant difference between testing media for C. albicans and mixed plaque (P= 0.078) or testing duration at 24 hours and 21 days (P= 0.07). Statistically significant differences were noted between all treatment solutions group combinations (P< 0.001), However, there was no difference between 30% Softsoap and Efferdent (P= 0.056).