Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Two-Step Electrophoretic Fabricated Sandwich-Structured CNT Cold Cathode With Defect-Coupled Multilevel NiO<sub>x</sub> for Superior Field Emission.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Immunoregulatory activity and metal-ion-binding properties of a glycoprotein isolated from mountain cultivated ginseng.

International immunopharmacology·2025

Same author

Amplification of Chirality in Self-Assembled Chiral Perovskite Superstructure Films.

Journal of the American Chemical Society·2025

Same author

Pushing the resolution limit of coherent diffractive imaging.

Light, science & applications·2025

Same author

Anomalous photoelectrochemical etching of undoped semiconductor surfaces.

Nature communications·2025

Same author

Effects of Substrate Temperature on Optical, Structural, and Surface Properties of Metal-Organic Vapor Phase Epitaxy-Grown MgZnO Films.

Nanomaterials (Basel, Switzerland)·2024

Same journal

Dual-mode switchable and reconfigurable Van der Waals phototransistor for multi-state image encryption.

Light, science & applications·2026

Same journal

Weak polarization electric field Ⅲ-N LEDs on polar plane with enhanced efficiency and strong lateral carrier confinement.

Light, science & applications·2026

Same journal

Bi-layer photonic random meta-composite for cryogenic thermal control by ultra-broadband scattering matched reflectance.

Light, science & applications·2026

Same journal

Interferometric scattering for optical tomoslicing of transparent solids.

Light, science & applications·2026

Same journal

Multi-dimensional spatial-temporal projection ultrafast compressed imaging.

Light, science & applications·2026

Same journal

Expanded field of view light-field extended-reality displays with metalens array.

Light, science & applications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 15, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Ultra-broadband diffractive imaging with unknown probe spectrum.

Chuangchuang Chen¹, Honggang Gu^2,3, Shiyuan Liu^4,5

¹State Key Laboratory of Intelligent Manufacturing Equipment and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.

Light, Science & Applications

|August 26, 2024

Summary

This summary is machine-generated.

This study introduces a new method for ultra-broadband coherent diffractive imaging (CDI) without needing to know the illumination spectrum. This advances computational imaging by enabling efficient photon use across wider spectral bands.

More Related Videos

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

Published on: May 11, 2014

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Related Experiment Videos

Last Updated: Jun 15, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

Published on: May 11, 2014

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Area of Science:

Optics and Photonics
Computational Imaging
Spectroscopy

Background:

Coherent diffractive imaging (CDI) traditionally requires a narrow, coherent spectrum, limiting photon utilization.
Existing broadband computational imaging methods necessitate precise spectroscopic measurements of the illumination source.
Broad spectra are generally incompatible with conventional CDI systems, posing a challenge for efficient imaging.

Purpose of the Study:

To develop an advanced approach for ultra-broadband CDI that accommodates an unknown probe spectrum.
To overcome the limitations of current broadband diffractive imaging frameworks.
To eliminate the need for prior spectroscopic knowledge and relax constraints on sample dispersion.

Main Methods:

Developed a novel computational imaging framework extending CDI to ultra-broadband illumination.
Implemented a method to monochromatize broadband diffraction patterns from unknown spectra.
Determined the compressive sampled spectrum of diffracted radiation.

Main Results:

Achieved a nearly fourfold improvement in bandlimit compared to existing benchmarks.
Demonstrated successful experimental validation using CDI and ptychography on an ultra-broadband supercontinuum (>40% relative bandwidth).
Showcased significantly enhanced coherence and high-fidelity reconstructions under ultra-broadband illumination.

Conclusions:

The new method broadens the spectral bandwidth usable in CDI significantly.
It removes the requirement for known probe spectra and non-dispersive samples.
This advancement enables more efficient photon utilization and higher quality imaging in broadband computational microscopy.