Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hyposaline LUSO Mineral Water Drives Murine Macrophage Polarization Towards an Anti-Inflammatory M2 Phenotype.

Food science & nutrition·2026

Same author

Rock surfaces as reservoirs for airborne halophilic microorganisms in the Bochnia Salt Mine.

Frontiers in microbiology·2026

Same author

Case Report: A Case of Refractory Polyarteritis Nodosa Treated With High-Dose Tofacitinib.

International journal of rheumatic diseases·2026

Same author

Airborne Halophilic and Non-Halophilic Microbial Communities in an Underground Salt Mine Affected by Seasonal Environmental Fluctuations.

Environmental microbiology reports·2026

Same author

Pregnancy and Pregnancy Outcomes in Women with Eating Disorders: A Four-Year Longitudinal Study with Case Series.

Pediatric reports·2025

Same author

Genome sequence of the marine bacterium <i>Roseobacter</i> sp. EG26, isolated from the octocoral <i>Eunicella gazella</i>, suggests aptitude for a host-associated lifestyle.

Microbiology resource announcements·2025

Search research articles

Related Experiment Video

Updated: Jun 15, 2025

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Multi-marker DNA metabarcoding for precise species identification in ichthyoplankton samples.

André O Ferreira^1,2, Olga M Azevedo^3,4, Cristina Barroso^5,6

¹Centre of Molecular and Environmental Biology (CBMA) and ARNET-Aquatic Research Network Associated Laboratory, Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal. alof446@gmail.com.

Scientific Reports

|August 26, 2024

Summary

This summary is machine-generated.

DNA metabarcoding significantly outperforms traditional morphology for identifying fish species in ichthyoplankton. This advanced method enhances accuracy and comprehensiveness in fish stock assessments.

More Related Videos

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

Related Experiment Videos

Last Updated: Jun 15, 2025

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Published on: November 25, 2016

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

Area of Science:

Marine Biology
Molecular Ecology
Fisheries Science

Background:

Ichthyoplankton monitoring is vital for effective fish stock assessments, providing data on spawning, recruitment, and biodiversity.
Traditional morphology-based identification methods can be time-consuming and may lack precision for larval fish stages.

Purpose of the Study:

To evaluate the efficiency of multi-marker DNA metabarcoding against morphology for fish species identification in ichthyoplankton.
To compare the performance of single versus multi-marker DNA metabarcoding approaches.
To assess the influence of sampling location and primer choice on species community composition.

Main Methods:

Ichthyoplankton samples were collected along the southern coast of Portugal and analyzed using both morphology and multi-marker DNA metabarcoding (COI, 12S, 16S rRNA).
DNA metabarcoding utilized multiple genetic markers to enhance species detection capabilities.
Statistical analyses, including PERMANOVA, were employed to compare community structures.

Main Results:

DNA metabarcoding identified 75 species compared to 11 by morphology, demonstrating superior performance.
Multi-marker metabarcoding increased species detection by 20-36% over single markers.
Significant differences in species communities were observed based on primer set, transect, and coastal distance.

Conclusions:

Multi-marker DNA metabarcoding offers a more accurate and comprehensive approach to ichthyoplankton species identification.
Integrating DNA metabarcoding into routine surveys can significantly improve fish stock assessments.
This molecular technique resolves uncertainties inherent in traditional identification methods.