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Assessing peri-implant bacterial community structure: the effect of microbiome sample collection method
Eduardo Anitua1,2, Alia Murias-Freijo3,4, Roberto Tierno5,3
1BTI-Biotechnology Institute, Vitoria, Spain. eduardo@fundacioneduardoanitua.org.
BMC Oral Health
|August 26, 2024
Summary
Choosing the right sampling method is crucial for accurately characterizing peri-implant biofilms. Different strategies significantly alter the detected bacterial community composition and structure, impacting research findings.
Area of Science:
- Microbiology
- Dental Implantology
- Biofilm Research
Background:
- Oral microbiota plays a key role in host homeostasis and immune function.
- Dysbiosis of oral bacteria is linked to various conditions, including oral cancer.
- Peri-implant diseases are inflammatory conditions associated with dental implant biofilms.
Purpose of the Study:
- To evaluate the impact of different sampling strategies on peri-implant biofilm characterization.
- To identify the most representative microbiome sampling procedure for peri-implant biofilms.
Main Methods:
- Next-generation sequencing was employed to analyze bacterial communities.
- Three distinct sampling methods were compared: adjacent periodontal crevicular fluid (ToCF), crevicular fluid from transepithelial abutment (TACF), and transepithelial abutment (TA).
- Samples were collected two months post-transepithelial abutment placement.
Main Results:
- Significant differences in alpha diversity indices (OTU and genus levels) were observed across sampling strategies.
- Differentially abundant taxa, including those related to peri-implant health and disease, were identified.
- Community composition varied significantly between TACF and TA, and between TA and ToCF.
Conclusions:
- The choice of sample collection strategy profoundly influences the detected composition and structure of peri-implant biofilms.
- Selecting appropriate sampling methods is essential for accurate biofilm characterization and understanding peri-implant diseases.
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