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Induction of apoptosis by oridonin in nonfunctioning pituitary adenoma cells

Hui-Tong Chen1, Xing-Yi Yuan1, Zhong-Yu Wang1

  • 1Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, China.

Drug Development Research
|August 27, 2024
PubMed

Insights

Oridonin (ORI) effectively inhibits nonfunctioning pituitary adenoma (NFPA) cell growth by disrupting the cell cycle and inducing apoptosis. This natural compound shows promise as a novel therapeutic agent for NFPA treatment.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Pharmacology

Background:

  • Nonfunctioning pituitary adenomas (NFPA) are common pituitary tumors with limited treatment options beyond surgery.
  • Current surgical interventions often fail to achieve complete tumor resection, necessitating the development of novel therapeutic agents.
  • Oridonin (ORI), a natural compound, has demonstrated antitumor properties in various cancers, but its efficacy against NFPA remains unexplored.

Purpose of the Study:

  • To investigate the therapeutic potential of Oridonin (ORI) against nonfunctioning pituitary adenomas (NFPA).
  • To elucidate the mechanisms by which ORI affects NFPA cell behavior, including proliferation, migration, and apoptosis.

Main Methods:

  • In vitro assays were employed to assess ORI's effects on pituitary-derived folliculostellate (PDFS) cells, including viability (CCK-8), colony formation, proliferation (EdU), migration (wound-healing), and invasion (Transwell).
  • Transcriptome sequencing, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify affected signaling pathways.
  • Cell cycle analysis, Annexin V/PI staining for apoptosis, and Western blot for apoptosis-related proteins (Bax, Bcl-2, cleaved Caspase-3) were conducted.

Main Results:

  • Oridonin significantly inhibited PDFS cell viability, proliferation, migration, and invasion.
  • Transcriptome analysis revealed that ORI impacts cell cycle and apoptosis pathways in PDFS cells.
  • ORI treatment led to G2/M phase cell cycle arrest and induced significant apoptosis.

Conclusions:

  • Oridonin effectively suppresses nonfunctioning pituitary adenoma cell growth through combined disruption of the cell cycle and induction of apoptosis.
  • ORI demonstrates significant potential as a novel therapeutic agent for nonfunctioning pituitary adenoma treatment.
Keywords:
apoptosisnonfunctioning pituitary adenomaoridonin

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