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Immunologic memory to phosphorylcholine. VI. Heterogeneity in light chain gene expression
European Journal of Immunology
|February 1, 1985
Summary
BALB/c mice produce two anti-PC antibody groups with distinct specificities. Group II antibodies utilize diverse VL genes, some related to Group I, showing varied antibody gene expression.
Area of Science:
- Immunology
- Molecular Biology
Background:
- BALB/c mice immunized with phosphorylcholine-conjugated keyhole limpet hemocyanin (PC-KLH) generate distinct anti-PC antibody populations.
- These antibodies, categorized as group I and group II, exhibit differences in fine specificity and idiotype expression.
Purpose of the Study:
- To investigate the genetic basis of anti-PC antibody diversity in BALB/c mice.
- To analyze the variable light (VL) chain gene usage in group II anti-PC hybridomas and serum antibodies.
Main Methods:
- Analysis of anti-PC hybridomas from BALB/c and (CBA/N x BALB/c)F1 male mice.
- Purification and isoelectric focusing analysis of anti-PC antibodies from BALB/c and C57BL/6N antisera.
Main Results:
- Isoelectric focusing confirmed V kappa 1-3 gene utilization in a subset of group II hybridomas and serum antibodies.
- Group II antibodies demonstrated diverse light chain expression, with some related to group I antibodies.
- One hybridoma was identified to utilize lambda 2 light chains.
Conclusions:
- Anti-PC antibodies with group II fine specificity employ a variety of VL genes.
- The VL gene repertoire for group II antibodies is broader and partially overlaps with that of group I antibodies.