Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lysosomes01:31

Lysosomes

15.9K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
15.9K
Non-equilibrium in the Cell01:16

Non-equilibrium in the Cell

4.0K
An important concept in studying metabolism and energy is that of chemical equilibrium. Most chemical reactions are reversible. They can proceed in both directions, releasing energy into their environment in one direction, and absorbing it from the environment in the other direction. The same is true for the chemical reactions involved in cell metabolism, such as the breaking down and building up of proteins into and from individual amino acids, respectively. Reactants within a closed system...
4.0K
Subcellular Fractionation01:32

Subcellular Fractionation

7.2K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
7.2K
Fixation and Sectioning01:03

Fixation and Sectioning

6.0K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
6.0K
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

9.0K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
9.0K
Structure and Function of Leukocytes01:21

Structure and Function of Leukocytes

5.1K
An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
White blood cells protect the body...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

mRNA-laden LNP-enabled in situ CAR-macrophage alleviates liver fibrosis via inhibiting activated HSCs and modulating the immune microenvironment.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Adipose Stem Cell-Derived Apoptotic Vesicles Attenuate Hypertrophic Scarring by Targeting the CDC20/WNT Signaling Pathway.

Biomedicines·2026
Same author

Apoptotic Vesicle Membrane-Mediated Targeted Endothelial Mitochondrial Transplantation-Clearance Therapy for Diabetic Wound Healing.

Research (Washington, D.C.)·2026
Same author

LAPTM5-dependent lipophagy enhances ferroptosis sensitivity in glioma cells.

Translational cancer research·2026
Same author

The structurally defined polysaccharide of Atractylodes macrocephala Koidz demonstrates the ability to alleviate cyclophosphamide-induced immunosuppression and promote the restoration of intestinal homeostasis in mice.

Food research international (Ottawa, Ont.)·2026
Same author

Validation of the Chinese version of the psychological safety in high-fidelity simulation scale: a culturally compatible methodological study.

BMC nursing·2025

Related Experiment Video

Updated: May 2, 2026

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.8K

Stem cells for organoids.

Shutong Qian1, Jiayi Mao1, Zhimo Liu1

  • 1Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.

Smart Medicine
|August 27, 2024
PubMed
Summary
This summary is machine-generated.

Organoids, 3D cell cultures mimicking organs, show promise in disease modeling and drug screening. This review explores stem cell organoid protocols, applications, and challenges like maturity and heterogeneity for improved clinical translation.

Keywords:
cell surface engineeringgene editingorganoidstem cell

More Related Videos

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.1K
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
09:48

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Published on: February 25, 2022

4.0K

Related Experiment Videos

Last Updated: May 2, 2026

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.8K
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.1K
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
09:48

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Published on: February 25, 2022

4.0K

Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Organoids are advanced 3D cell culture models replicating organ structures and functions.
  • They are crucial for disease modeling, drug screening, and studying developmental biology.
  • Stem cell-derived organoids offer a powerful in vitro platform for organ culture.

Purpose of the Study:

  • To review current stem cell-derived organoid culturing protocols and applications.
  • To identify and address challenges in organoid development, such as maturity and heterogeneity.
  • To propose strategies for engineering stem cells for enhanced organoid research and clinical translation.

Main Methods:

  • Literature review of organoid culturing techniques and applications.
  • Analysis of stem cell differentiation and self-renewal within 3D matrices.
  • Identification of limitations in current organoid models.

Main Results:

  • Organoids provide a 3D environment supporting stem cell differentiation and self-renewal.
  • Current organoid models face challenges including low maturity, high heterogeneity, and lack of spatiotemporal control.
  • Significant progress has been made in various applications, including disease modeling and drug screening.

Conclusions:

  • Stem cell-derived organoids are valuable tools with broad applications.
  • Overcoming challenges in maturity, heterogeneity, and regulation is key for advancement.
  • Engineering stem cells holds potential for improved organoid models and clinical translation.