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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

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Published on: July 19, 2021

Freezing biological organisms for biomedical applications.

Gaizhen Kuang^1,2, Qingfei Zhang², Jinxuan Jia²

¹Pharmaceutical Sciences Laboratory Åbo Akademi University Turku Finland.

|August 27, 2024

Summary

Freezing biological organisms using cryoablation and cryopreservation offers new therapeutic strategies. These methods are crucial for inactivating pathogens and preserving living organisms for various biomedical applications, including disease treatment.

Keywords:

biological organisms cryoablation cryopreservation tissue regeneration transplantation

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Last Updated: Jun 15, 2025

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

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Published on: July 19, 2021

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

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Freezing, Thawing, and Packaging Cells for Transport

Freezing, Thawing, and Packaging Cells for Transport

Published on: July 2, 2008

Area of Science:

Biomedical Science
Microbiology
Cryobiology

Background:

Biological organisms significantly impact human health, acting as commensals or pathogens.
Harnessing inactivated or living organisms presents a promising avenue for disease treatment.
Current challenges include organism inactivation and long-term storage for therapeutic use.

Purpose of the Study:

To review the latest advancements in freezing biological organisms for biomedical applications.
To illustrate freezing strategies, specifically cryoablation and cryopreservation, and their technical aspects.
To discuss the challenges and future prospects of using freezing methods in organism-based therapies.

Main Methods:

Review of current scientific literature on freezing biological organisms.
Detailed explanation of cryoablation techniques for organism inactivation.
Explanation of cryopreservation techniques for long-term storage of living organisms.

Main Results:

Cryoablation effectively inactivates organisms, rendering them non-pathogenic for therapeutic use.
Cryopreservation provides a viable solution for the long-term storage of living organisms in therapies.
Freezing methods are applicable to transplantation, tissue regeneration, anti-infection, and anti-tumor therapies.

Conclusions:

Freezing techniques like cryoablation and cryopreservation are vital for organism-based therapies.
These methods offer potential standardization and commercialization pathways for therapeutic systems.
Advancements in freezing technology are expected to promote wider clinical application of organism-based treatments.