The effector SJP3 interferes with pistil development by sustaining SHORT VEGETATIVE PHASE 3 expression in jujube
Mingsheng Deng1,2, Fuli Ma1, Liping Zhai1
1Anhui Province Key Laboratory of Horticultural Crop Quality Biology, College of Horticulture, Anhui Agricultural University, 130 West Changjiang Road, Hefei City 230036, Anhui Province, People's Republic of China.
Plant Physiology
|August 27, 2024
Summary
Jujube witches
Area of Science:
- Plant Pathology
- Molecular Biology
- Genetics
Background:
- Jujube witches' broom (JWB) is a phytoplasma disease damaging jujube crops.
- Phytoplasmas cause floral reversion, leading to phyllody and vegetative growth.
- The secreted JWB protein 3 (SJP3) is known to induce phyllody.
Purpose of the Study:
- To elucidate the molecular mechanisms of SJP3-mediated pistil reversion in jujube.
- To identify proteins interacting with SJP3 and their role in disease development.
Main Methods:
- Investigated the interaction between SJP3 and ZjSVP3 using transgenic calli.
- Analyzed gene expression patterns in healthy and infected jujube tissues.
- Performed heterologous expression in *Nicotiana benthamiana* to study ZjSVP3 function.
- Examined promoter binding of ZjSVP3 to target genes ZjZFP8 and ZjSHP1.
Main Results:
- SJP3 interacts with and stabilizes the MADS-box protein ZjSVP3, preventing its degradation.
- ZjSVP3 expression is constitutively maintained in JWB-infected flowers.
- Heterologous expression of ZjSVP3 induced pistil abnormalities.
- ZjSVP3 directly regulates the expression of ZjZFP8 and ZjSHP1, affecting pistil development.
Conclusions:
- SJP3 effector stabilizes ZjSVP3, sustaining its expression and disrupting normal pistil development.
- This interaction provides a molecular mechanism for JWB phytoplasma-induced pistil reversion in jujube.
- Findings offer insights into phytoplasma-plant interactions in perennial woody species.
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