Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

385
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
385

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Turn-on Coumarin Precursor: From Hydrazine Sensor to Covalent Inhibition and Fluorescence Detection of Rabbit Muscle Aldolase.

Molecules (Basel, Switzerland)·2024
Same author

Shining light on fluoride detection: a comprehensive study exploring the potential of coumarin precursors as selective turn-on fluorescent chemosensors.

Organic & biomolecular chemistry·2023
Same author

Thioxobimanes.

The Journal of organic chemistry·2023
Same author

Detection of anabolic steroids <i>via</i> cyclodextrin-promoted fluorescence modulation.

RSC advances·2022
Same author

A dipodal bimane-ditriazole-diCu(II) complex serves as an ultrasensitive water sensor.

Chemical communications (Cambridge, England)·2022
Same author

Sonication-Induced, Solvent-Selective Gelation of a 1,8-Napthalimide-Conjugated Amide: Structural Insights and Pollutant Removal Applications.

ACS omega·2021

Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

2.2K

Ultrasensitive and versatile hydrogen peroxide sensing via fluorescence quenching.

Jenisha John Peter1, Nathaniel Chennattuparambil Roy1, Flavio Grynszpan1

  • 1Department of Chemical Sciences, Ariel University, 65 Ramat HaGolan Street, Ariel 40700, Israel. mindyl@ariel.ac.il.

Chemical Communications (Cambridge, England)
|August 27, 2024
PubMed
Summary

A novel fluorescence sensor detects hydrogen peroxide with ultra-high sensitivity. This highly efficient sensor works in solution or vapor phases, even on paper.

More Related Videos

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
05:16

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Published on: January 19, 2024

4.4K
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
04:53

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

30.2K

Related Experiment Videos

Last Updated: Jun 15, 2025

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

2.2K
Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
05:16

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines

Published on: January 19, 2024

4.4K
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
04:53

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

30.2K

Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Materials Science

Background:

  • Hydrogen peroxide (H2O2) is a key biomarker in various biological and environmental processes.
  • Accurate detection of H2O2 is crucial for diagnostics and monitoring.
  • Existing H2O2 sensors often lack sensitivity or versatility.

Purpose of the Study:

  • To develop an ultra-sensitive fluorescence sensor for hydrogen peroxide detection.
  • To investigate the sensor's performance in different detection modes (solution and vapor).
  • To assess the sensor's stability and applicability on a solid support like paper.

Main Methods:

  • Utilized the highly efficient reaction between bimane and hydrogen peroxide.
  • Developed a turn-off fluorescence sensing mechanism.
  • Immobilized the sensor onto paper for solid-state detection.
  • Evaluated sensor performance using fluorescence spectroscopy.

Main Results:

  • Achieved an ultra-low detection limit of 7.9 picomolar (pM) for hydrogen peroxide.
  • Demonstrated high sensitivity and efficiency in the fluorescence quenching reaction.
  • Confirmed sensor functionality in both solution-state and vapor-phase detection.
  • Maintained sensor efficacy when adsorbed onto paper.

Conclusions:

  • The developed bimane-based fluorescence sensor offers unprecedented sensitivity for hydrogen peroxide.
  • The sensor's adaptability to paper supports enables portable and field-deployable H2O2 detection.
  • This technology holds promise for sensitive and versatile hydrogen peroxide monitoring in diverse applications.