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Analyte Adsorption and Distribution

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...

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Related Experiment Video

Updated: Jun 15, 2025

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Assay for Characterizing Adsorption-Properties of Surfaces (APS).

Bente Siebels¹, Manuela Moritz¹, Diana Hübler²

¹Section Mass Spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf, Martininstr. 52, Hamburg, 20246, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|August 27, 2024

Summary

Analyte adsorption to surfaces during sample prep causes sample loss. This new assay quantifies adsorption interactions, helping to minimize analyte loss and improve omics data accuracy.

Keywords:

Adsorption-dependent loss of molecules Interaction of solubilized molecules with surfaces LC–MS/MS based label-free quantification

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Analyte adsorption to surfaces during sample preparation is a significant challenge in quantitative analysis, particularly in omics studies.
Existing methods like internal standards are insufficient for minimizing overall analyte loss across diverse molecules.
Understanding and quantifying adsorption interactions is crucial for improving analytical accuracy.

Purpose of the Study:

To develop and validate a novel assay for qualifying and quantifying adsorption of molecules to surfaces (APS).
To assess the adsorption properties of various vial surfaces using a comprehensive peptide mixture.
To provide a method for approximating analyte loss during sample preparation.

Main Methods:

Utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based differential quantitative analysis.
Employed a reference mixture of thousands of tryptic peptides with diverse chemical characteristics.
Investigated adsorption properties of different vial types, including conventional polypropylene, low-protein-binding polypropylene, and glass vials.

Main Results:

Demonstrated significant adsorption of hydrophobic peptides to conventional polypropylene vials.
Observed minimal peptide adsorption to low-protein-binding polypropylene vials.
Identified substantial peptide adsorption to glass vials, primarily driven by electrostatic interactions.

Conclusions:

The developed assay effectively characterizes the adsorption properties of different surfaces.
The assay can approximate analyte loss during sample preparation, aiding in method optimization.
Findings highlight the impact of surface chemistry on analyte recovery and suggest appropriate vial selection for omics studies.