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Complete genome sequences of Clostridium perfringens CP201 isolated from an asymptomatic rhesus monkey (Macaca

Sung Guk Kim1, Sharon X Wang1, Miseon Park2

  • 1Surveillance/Diagnostic Laboratory, Office of Scientific Coordination, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas, USA.

Microbiology Resource Announcements
|August 27, 2024
PubMed

Insights

The genome of Clostridium perfringens CP201, isolated from a rhesus monkey, was sequenced. This analysis provides insights into the genetic makeup of this bacterium, a common cause of human gastroenteritis.

Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Science

Background:

  • Clostridium perfringens is a significant pathogen causing human gastroenteritis.
  • Understanding the genetic diversity of C. perfringens is crucial for public health.
  • Non-human primates can serve as reservoirs for bacterial pathogens.

Purpose of the Study:

  • To sequence and characterize the genome of Clostridium perfringens CP201 from an asymptomatic rhesus monkey (Macaca mulatta).
  • To provide a genomic resource for studying C. perfringens evolution and pathogenicity.
  • To investigate potential zoonotic implications of C. perfringens strains in non-human primates.

Main Methods:

  • Whole-genome sequencing of Clostridium perfringens CP201.
  • Bioinformatic analysis of the chromosomal and plasmid DNA.
  • Identification and annotation of genes, tRNAs, rRNAs, and CRISPR arrays.

Main Results:

  • The genome comprises a chromosome (3,241,413 bp) and a plasmid (92,983 bp).
  • The genome encodes 2,935 genes, 94 tRNAs, 30 rRNAs, and 1 CRISPR array.
  • GC content for the chromosome and plasmid were 30.22% and 28.43%, respectively.

Conclusions:

  • The genome sequence of C. perfringens CP201 provides a valuable reference for comparative genomics.
  • This study highlights the potential of non-human primates as reservoirs for C. perfringens.
  • Further research is warranted to assess the public health significance of C. perfringens strains from animal hosts.
Keywords:
Clostridiumperfringensrhesus monkey

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