Versatile, reusable and highly sensitive SERS-based point-of-care testing microplatform for reliable ATP detection

Area of Science:

• Nanotechnology
• Analytical Chemistry
• Biomedical Engineering

Background:

• Miniaturized Raman spectrometers and surface-enhanced Raman scattering (SERS) show promise for point-of-care testing (POCT).
• Accurate quantification of analytes in complex biological samples at low volumes remains a challenge.
• Adenosine triphosphate (ATP) is a crucial biomarker in various biological processes.

Purpose of the Study:

• To develop a versatile and reusable SERS microplatform for sensitive and reliable quantitative detection of ATP in biological fluids.
• To overcome limitations in analyte quantification within complex matrices and limited sample volumes.
• To establish a robust method for ATP detection suitable for POCT.

Main Methods:

• Fabrication of a SERS microplatform using gold-Prussian blue core-shell nanoparticles (Au@PB@PEI NPs) embedded in silica monoliths.
• Utilizing Prussian blue (PB) as an internal standard and polyethyleneimine (PEI) for enhanced molecular capture.
• Employing magnesium ions for regulated ATP capture/release and ratiometric signal analysis for quantification.

Main Results:

• Achieved a limit of detection for ATP down to 0.62 pM.
• Demonstrated high reproducibility and accuracy in quantifying ATP in mouse cerebrospinal fluid and human serum.
• The platform enabled rapid analysis (approximately 10 minutes) with minimal matrix interference.

Conclusions:

• The developed SERS microplatform offers a sensitive, accurate, and reusable solution for ATP quantification.
• The platform's design integrates efficient enrichment, separation, and internal calibration, suitable for POCT.
• This technology holds significant potential for advancing diagnostic capabilities in biological fluid analysis.