Gravity-assisted gradient size exclusion separation of microparticles by gap-modifiable silicon nanowire arrays

The separation and detection of microparticles within complex samples pose substantial challenges due to the intricate variations in size and concentration. A strategy employing gravity-assisted gradient size exclusion principle based on controllable gap sizes on the surface of silicon nanowire arrays (SiNWAs) has been established to achieve the separation of microparticles with diverse sizes. The formation of gradient gap sizes was accomplished by meticulously investigating the impact of oxidation-reduction reactions through metal-assisted chemical etching. Particles of different sizes were initially aggregated at the accumulation base, followed by a sequential size exclusion process within the finely regulated 0.9-12.5 μm gradient-gap-sized separation region facilitated with gravity-assisted, leading to a comprehensive separation of microparticles based on their respective size differences, progressing from small to large. The effective separation of four model-sized microparticles demonstrated a separation degree of ≥2.7, purity of ≥96.1 %, RSDs of ≤4.6 %, and a separation capacity of up to 10⁷ particles. The separation efficacy of this gradient-sized chip was verified by evaluating the more complex atmospheric particulates with varying sizes, which exhibited separation degree ranging between 2 and 10. This method offers a precise separation range, easily adjustable separation sizes, and simple operation, rendering it a versatile tool for separating complex samples.