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Updated: Jul 26, 2026

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
Published on: August 30, 2013
Reconsidering Neurogenetic Indication in the Human Brain: Broad Expression of Doublecortin Transcript in the
Tae-Hyeon Cho1,2, Miri Kim2,3, Shin Hyung Kim2,4
1Department of Anatomy, College of Korean Medicine, Semyung University, Jecheon, Republic of Korea.
Introduction:
Neurogenesis in the adult brain may play an important role in memory and cognition; however, knowledge of neurogenic markers in the human brain remains limited. We compared the single-nucleus transcriptome of the hippocampus with that of other cortical regions to identify hippocampus-specific neurogenic markers.
Methods:
We analyzed 26,189 nuclei from four human brains collected within 16 h of death. Clustering and annotation were performed to examine differential expression, gene ontology, and intercellular communication. DCX expression was validated by ddPCR.
Results:
Immature markers such as DCX, CALB2, NES, SOX2, PAX6, DPYSL3, and TUBB3 were expressed in both hippocampus and prefrontal cortex, with higher levels in the prefrontal cortex. ddPCR confirmed higher expression of DCX in the prefrontal cortex. DCX was involved in both neurogenesis and neuroprotection pathways.
Conclusion:
Neurogenic markers are not definitive indicators of adult neurogenesis as their roles are more complex than previously understood.
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