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Related Experiment Video

Updated: Jun 15, 2025

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Twisted cell flow facilitates three-dimensional somite morphogenesis in zebrafish.

Harunobu Kametani1, Yue Tong1, Atsuko Shimada1

  • 1Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo, Tokyo 113-0033, Japan.

Cells & Development
|August 27, 2024
PubMed
Summary

Zebrafish somite elongation involves complex, twisted cell flows. Sdf1 signaling is crucial for this cell movement and tissue shaping during embryonic development.

Keywords:
Collective cell migrationSdf1 signalingSomite elongationWhole-somite rotationZebrafish

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biophysics

Background:

  • Tissue elongation is vital for forming complex embryonic structures.
  • Zebrafish somites elongate rapidly, changing from cuboidal to V-shapes.
  • Cellular mechanisms driving somite elongation lack single-cell resolution data.

Purpose of the Study:

  • To investigate single-cell behaviors during zebrafish somite elongation.
  • To elucidate the role of cell movement and signaling in tissue morphogenesis.
  • To understand the contribution of cell dynamics to embryonic development.

Main Methods:

  • Utilized lightsheet microscopy to track cell motion and shapes in 3D.
  • Performed chemical inhibition studies targeting Sdf1 signaling.
  • Employed 3D computational modeling to analyze cell dynamics.

Main Results:

  • Identified simultaneous horizontal and dorsal cell movements creating a twisted flow.
  • Discovered Sdf1 signaling inhibition disrupts cell flow and somite elongation.
  • Computational models indicated horizontal cell rotation drives dorsoventral elongation.

Conclusions:

  • Collective cell migration, characterized by twisted flow, drives somite elongation.
  • Sdf1 signaling is essential for coordinating cell movements in somite morphogenesis.
  • Cellular dynamics in 3D space are critical for embryonic tissue development.