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Updated: Jun 15, 2025

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Click Chemistry in Detecting Protein Modification.

Chaoqun Li¹, Mutian Jia²

¹Key Laboratory of Infection and Immunity of Shandong Province & Department of Immunology, School of Basic Medical Science, Cheeloo College of Medicine, Shandong University, Jinan, China.

Methods in Molecular Biology (Clifton, N.J.)

|August 27, 2024

Summary

This summary is machine-generated.

Click chemistry enables efficient molecular connections, proving valuable for studying protein posttranslational modifications (PTMs) during viral infections. This method details the analysis of STING protein modifications like palmitoylation, carbonylation, and alkylation.

Keywords:

Alkynylated probes Cell metabolism Click chemistry PTMs

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Detection of Protein Ubiquitination

Detection of Protein Ubiquitination

Published on: August 19, 2009

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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

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Detection of Protein Ubiquitination

Detection of Protein Ubiquitination

Published on: August 19, 2009

Area of Science:

Biochemistry
Molecular Biology
Chemical Biology

Background:

Click chemistry is a powerful technique for creating molecular linkages with high efficiency and selectivity.
Protein posttranslational modifications (PTMs) are crucial for cellular function and are often altered during viral infections.
Understanding PTMs provides insights into cellular responses to pathogens.

Purpose of the Study:

To highlight the utility of click chemistry in analyzing PTMs.
To present methodologies for measuring specific PTMs on the STING protein.
To explore the role of PTMs in viral infection contexts.

Main Methods:

Utilizing click chemistry for facile and specific conjugation reactions.
Applying click chemistry to detect and quantify PTMs such as palmitoylation, carbonylation, and alkylation.
Detailed experimental procedures for PTM analysis on STING.

Main Results:

Demonstrated the efficiency and selectivity of click chemistry for PTM analysis.
Provided a framework for measuring specific PTMs on STING.
Established click chemistry as a viable tool for studying virus-induced metabolic changes affecting PTMs.

Conclusions:

Click chemistry is an effective strategy for the in-depth analysis of protein PTMs.
The presented methods enable precise measurement of STING modifications.
This approach facilitates research into the interplay between cell metabolism, viral infection, and protein modifications.