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Updated: Jun 15, 2025

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
Protein Kinase R (PKR) as a Novel dsRNA Sensor in Antiviral Innate Immunity
Huibin Yu1,2, Dewi Megawati1, Chunfu Zheng3
1Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.
Abstract:
Protein kinase R (PKR), a key double-stranded RNA (dsRNA)-activated sensor, is pivotal for cellular responses to diverse stimuli. This protocol delineates a comprehensive methodological framework employing single luciferase assays, yeast assays, immunoblot assays, and quantitative PCR (qPCR) to discern and validate PKR activities and their downstream impacts on NF-κB-activating signaling pathways. These methodologies furnish a systematic approach to unraveling the role of PKR as a dsRNA sensor and effector in antiviral innate immunity, enabling in-depth analyses of dsRNA sensor activities.
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