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Potential mechanism prediction of indole-3-propionic acid against diminished ovarian reserve via network pharmacology, molecular docking and experimental verification

  • 0The First School of Clinical Medicine, Lanzhou University, Lanzhou, Gansu, China.
Bmc Complementary Medicine and Therapies +

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August 27, 2024

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August 27, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Indole-3-propionic acid (IPA) protects ovarian reserve by combating oxidative stress through multiple targets and pathways. In vitro studies confirm IPA’s cytoprotective and antioxidant effects, offering new insights into treating diminished ovarian reserve (DOR).

Area Of Science

  • Reproductive biology
  • Pharmacology
  • Biochemistry

Background

  • Oxidative stress (OS) is a primary driver of ovarian aging and dysfunction.
  • Indole-3-propionic acid (IPA), a tryptophan derivative, possesses antioxidant properties.
  • The precise mechanisms by which IPA protects ovarian function remain largely unknown.

Purpose Of The Study

  • To elucidate the protective mechanisms of IPA against diminished ovarian reserve (DOR).
  • To investigate IPA's effects on ovarian function using network pharmacology, molecular docking, and in vitro validation.

Main Methods

  • Network pharmacology identified IPA targets and DOR-related genes.
  • Protein-protein interaction (PPI) networks and pathway analyses (GO, KEGG) were performed.
  • Molecular docking and in vitro experiments validated IPA's interactions and effects.

Main Results

  • Identified 61 common targets for IPA and DOR, with 13 key hub genes.
  • IPA demonstrated binding with hub proteins (NOS3, AKT1, EGFR, PPARA, SRC, TNF).
  • In vitro experiments confirmed IPA's attenuation of oxidative stress and cytoprotective effects.

Conclusions

  • IPA exhibits potential protective effects against DOR via multiple targets and pathways.
  • Network pharmacology and in vitro studies confirm IPA's antioxidant and cytoprotective properties.
  • Findings offer novel insights into IPA's molecular mechanisms for improving DOR.

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