A proposal to improve calibration and outlier detection in high-throughput mass spectrometry
Adam P R Zabell1, Fred E Lytle1, Randall K Julian1
1Indigo BioAutomation, 7820 Innovation Blvd, Suite 250, Indianapolis, IN 46278, United States.
Abstract:
Instrument calibration, required for any accurate quantitative calculation, is a trivial process when performed correctly, but is also full of easily overlooked stumbling blocks. To minimize the risk of error associated with improper calibrations, national and international guidance dictates a minimum number of calibrators and the threshold at which a measurement becomes an outlier. Evidence from industry practice, which conflicts with regulatory guidance, suggests that most groups are focused on remapping their detector with each run. We present a post facto explanation for the calibrator minimum and provide recommendations for curve building, which include improved outlier detection for high-volume mass spectrometry laboratories.
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