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Published on: October 24, 2018
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Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry
Yuming Jiang1,2,3, Devasahayam Arokia Balaya Rex4, Dina Schuster5,6,7
1Department of Computational Biomedicine, Cedars Sinai Medical Center, Los Angeles, California 90048, United States.
ACS Measurement Science Au
|August 28, 2024
Summary
This review provides a comprehensive guide to bottom-up proteomics, covering methods from protein extraction to data interpretation. It serves as a handbook for researchers new to large-scale protein analysis and quantification.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Proteomics involves large-scale study of protein structure and function.
- Bottom-up proteomics, a common strategy, analyzes peptides derived from proteins using mass spectrometry.
- Diverse applications exist, including protein identification, quantification, and analysis of modifications.
Purpose of the Study:
- To provide a comprehensive overview of different proteomics methods.
- To clarify the nuances of various proteomic workflows for new researchers.
- To serve as a handbook for practitioners entering bottom-up proteomics.
Main Methods:
- Overview of biochemical principles relevant to proteomics.
- Detailed explanation of protein extraction techniques.
- Discussion of mass spectrometry-based peptide analysis.
- Guidance on biological interpretation and orthogonal validation.
Main Results:
- The review covers a wide spectrum of proteomic analysis strategies.
- It addresses challenges faced by new researchers in understanding proteomic workflows.
- It integrates biochemical foundations with advanced analytical techniques.
Conclusions:
- This review offers a foundational understanding of bottom-up proteomics.
- It equips researchers with knowledge for diverse proteomic experiments.
- It aims to facilitate entry and proficiency in the field of proteomics.
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