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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Antibacterial activity of a silver-incorporated vancomycin-modified mesoporous silica against methicillin-resistant

Mehdi Chamani1, Shadi Asgari2, Ali Najmeddin1

  • 1Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Journal of Biomaterials Applications
|August 28, 2024
PubMed

Insights

New silver and vancomycin nanoparticles effectively detect and treat methicillin-resistant Staphylococcus aureus (MRSA) infections. This nanomedicine shows enhanced antibacterial activity and reduces inflammation, offering a promising alternative to conventional antibiotics for MRSA.

Area of Science:

  • Nanotechnology
  • Materials Science
  • Infectious Diseases

Background:

  • Conventional antibiotics are losing efficacy against methicillin-resistant Staphylococcus aureus (MRSA).
  • Vancomycin is used for MRSA detection, but novel therapeutic strategies are needed.
  • Mesoporous silica nanoparticles (MSNs) offer a versatile platform for drug delivery and modification.

Purpose of the Study:

  • To design and characterize silver-incorporated vancomycin-modified mesoporous silica nanoparticles (MSNs@Van@Ag NPs).
  • To evaluate the antibacterial efficacy of MSNs@Van@Ag NPs against MRSA in vitro and in vivo.
  • To assess the safety and anti-inflammatory effects of MSNs@Van@Ag NPs.

Main Methods:

  • MSNs were synthesized and modified with vancomycin, followed by incorporation of silver nanoparticles (Ag NPs).
  • Characterization included size, surface area, and thermal stability analysis.
  • In vitro antibacterial susceptibility tests (MIC) and in vivo acute pneumonia models were employed.

Main Results:

  • MSNs@Van@Ag NPs exhibited a spherical shape (50-100 nm) with high surface area (955.8 m²/g) and thermal stability.
  • Silver release was sustained (75% over 100 h).
  • MSNs@Van@Ag NPs demonstrated enhanced antibacterial activity (MIC of 50 μg/mL) compared to MSNs@Van (MIC of 100 μg/mL) and reduced pro-inflammatory cytokines in vivo.

Conclusions:

  • MSNs@Van@Ag NPs are effective in detecting and treating MRSA infections.
  • The nanomedicine exhibits enhanced antibacterial properties and a favorable safety profile for in vivo applications.
  • This novel nanomedicine alleviates inflammatory responses, presenting a potential alternative to conventional antibiotics.
Keywords:
Mesoporous silicaantibacterial activitymethicillin-resistant staphylococcus aureussilvervancomycin

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