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Updated: Jun 15, 2025
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Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
Published on: July 15, 2015
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Impact of device variability and protocol differences on kidney function during normothermic machine perfusion: A
Veerle A Lantinga1, Asel S Arykbaeva2, Nora A Spraakman3
1Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
Artificial Organs
|August 28, 2024
Summary
Different kidney perfusion devices and protocols significantly impact organ function during normothermic machine perfusion (NMP). Careful consideration of device settings is crucial for optimal outcomes in NMP for kidney preservation.
Area of Science:
- Nephrology
- Transplantation
- Medical Devices
Background:
- Increasing clinical availability of renal normothermic machine perfusion (NMP) devices.
- Significant variations exist among NMP systems in circuits, pumps, sensors, and software.
Purpose of the Study:
- To evaluate the influence of different perfusion protocols and devices on kidney function and perfusion parameters during NMP.
Main Methods:
- Porcine kidneys underwent warm ischemia and cold storage before 6-hour perfusion with Kidney Assist (KA), modified KA, or Perlife (PL) devices.
- Human kidneys were subsequently perfused using KA or modified KA protocols.
Main Results:
- Perlife (PL) group exhibited lower pressure and arterial pO2 but higher oxygen consumption and increased hemoglobin concentration compared to KA groups.
- Modified KA in human kidneys showed reduced vascular resistance, enhanced oxygen delivery, and lower lactate levels versus KA.
- No significant differences were observed in tissue ATP, urine production, or creatinine clearance across groups.
Conclusions:
- Perfusion characteristics and kidney function are significantly affected by the specific NMP device and protocol used.
- Results emphasize the importance of interpreting NMP data with caution, considering device-specific parameters.
