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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 15, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

A Miniature Modular Fluorescence Flow Cytometry System.

Shaoqi Huang¹, Jiale Li¹, Li Wei¹

¹Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.

|August 28, 2024

Summary

This summary is machine-generated.

We developed a flexible modular fluorescence flow cytometry (M-FCM) system. This cost-effective, compact system simplifies fluorescence channel adjustment and offers multifunctional measurement potential for cell analysis.

Keywords:

drug screening flow cytometry fluorescence detection modular microfluidics

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Last Updated: Jun 15, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Published on: May 5, 2016

Area of Science:

Biophysics
Analytical Chemistry
Cell Biology

Background:

Traditional fluorescence flow cytometry systems are complex and difficult to adjust.
There is a need for more flexible and user-friendly cell analysis instruments.

Purpose of the Study:

To develop a modular fluorescence flow cytometry (M-FCM) system with flexible fluorescence channel arrangement.
To demonstrate the system's capability for cell viability assessment in drug screening.

Main Methods:

Development of modular particle focusing and fluorescence detection units.
Integration of in situ illumination and fluorescence detection.
Hydrodynamical focusing for cell measurement.

Main Results:

Achieved a signal-to-noise ratio of 33.2 dB.
Eliminated crosstalk among fluorescence channels.
M-FCM results for cell viability in drug screening showed good agreement with commercial systems.

Conclusions:

The M-FCM system offers flexibility, cost-efficiency, and ease of operation.
It provides a multifunctional platform for various biophysical measurements.
This modular approach enables innovative multivariate analysis of cellular characteristics.