Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-dimensional Dirac semimetal OD-BC<sub>3</sub> as an ultrahigh-performance anode for Li-, Na-, and K-ion batteries.

Physical chemistry chemical physics : PCCP·2026
Same author

The Potential of <i>Siraitia grosvenorii</i> to Promote Bone Regeneration via Modulating Macrophage Polarization: A Network Pharmacology and Experimental Study.

International journal of molecular sciences·2025
Same author

In-Situ Grafting Strategy Enables Functional Separator for Advanced Lithium-Sulfur Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

Multi-Scale Characterization of Conventional and Immediate Dental Implant Systems.

Journal of functional biomaterials·2024
Same author

Artificial Intelligence for Predicting the Aesthetic Component of the Index of Orthodontic Treatment Need.

Bioengineering (Basel, Switzerland)·2024
Same author

Effectiveness of Machine Learning in Predicting Orthodontic Tooth Extractions: A Multi-Institutional Study.

Bioengineering (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jun 15, 2025

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

12.9K

Osteoclast-Driven Polydopamine-to-Dopamine Release: An Upgrade Patch for Polydopamine-Functionalized Tissue

Lufei Wang1, Huamin Hu2, Ching-Chang Ko3

  • 1Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction & College and Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China.

Journal of Functional Biomaterials
|August 28, 2024
PubMed
Summary

Polydopamine scaffolds are functionalized for bone tissue engineering. Osteoclasts metabolize polydopamine to dopamine, promoting bone regeneration and offering a new strategy for scaffold development.

Keywords:
bone regenerationbone tissue engineeringdopaminedrug releaseosteoclasts

More Related Videos

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
09:19

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Published on: December 8, 2017

14.6K
Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold
09:37

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Published on: October 23, 2015

12.6K

Related Experiment Videos

Last Updated: Jun 15, 2025

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
05:49

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Published on: April 13, 2018

12.9K
Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
09:19

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Published on: December 8, 2017

14.6K
Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold
09:37

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Published on: October 23, 2015

12.6K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Research

Background:

  • Polydopamine (PDA) is a versatile polymer for functionalizing tissue engineering scaffolds due to its adhesive properties.
  • PDA-functionalized scaffolds show promise in preclinical bone tissue engineering.
  • The biodegradation, metabolism, and potential side effects of PDA metabolites during bone healing are largely unknown, hindering clinical translation.

Purpose of the Study:

  • To investigate the biodegradation and metabolic fate of polydopamine within bone tissue engineering scaffolds.
  • To identify the specific cells and mechanisms responsible for polydopamine metabolism.
  • To evaluate the biological effects of polydopamine metabolites on bone regeneration.

Main Methods:

  • Utilized a bioinspired polydopamine-laced hydroxyapatite collagen calcium silicate material as a model scaffold.
  • Investigated the interaction of osteoclasts with the polydopamine-functionalized scaffold.
  • Assessed the osteoinductive effects of released dopamine in vitro and its impact on bone regeneration in vivo using calvarial critical-sized defects.

Main Results:

  • Discovered that osteoclasts specifically metabolize polydopamine into dopamine, termed "osteoclast-driven polydopamine-to-dopamine release".
  • The released dopamine demonstrated significant osteoinductive effects in vitro.
  • Promoted substantial bone regeneration in calvarial critical-sized defects.

Conclusions:

  • Osteoclast-driven polydopamine-to-dopamine release is a novel mechanism for enhancing scaffold bioactivity.
  • This mechanism can be applied to existing polydopamine-functionalized scaffolds by recruiting osteoclasts, upgrading their osteoinductive potential.
  • This strategy offers a promising approach to improve bone regeneration and advance the clinical application of polydopamine-based materials.