Pogostemon cablin Extract Promotes Wound Healing through OR2AT4 Activation and Exhibits Anti-Inflammatory Activity

Area of Science:

• Dermatology
• Pharmacology
• Cell Biology

Background:

• Wound healing is a complex biological process involving four distinct phases.
• Chronic wounds result from persistent inflammation, impeding healing.
• The exact mechanisms of Pogostemon cablin extract (PCE) in wound healing and inflammation are not fully understood.

Purpose of the Study:

• To investigate the effects of PCE on human keratinocyte proliferation and wound healing.
• To evaluate the anti-inflammatory activity of PCE in vitro.
• To elucidate the molecular pathways involved in PCE's action, including OR2AT4 activation.

Main Methods:

• In vitro experiments using human keratinocytes (HaCaT) and THP-1 cells.
• Assessing cell proliferation and Ki67 expression.
• Measuring pro-inflammatory cytokine levels (interleukin-6 and -8) after stimulation with lipopolysaccharide and TNF-α.

Main Results:

• PCE significantly increased human keratinocyte proliferation and Ki67 expression.
• PCE accelerated wound healing in vitro, mediated by OR2AT4 activation.
• PCE reduced levels of interleukin-6 and -8 in stimulated immune cells, indicating anti-inflammatory effects.

Conclusions:

• PCE demonstrates therapeutic potential for wound healing by enhancing cell proliferation and accelerating the healing process.
• PCE possesses anti-inflammatory properties, making it a promising candidate for managing chronic wounds.
• The findings highlight PCE's ability to modulate key pathways involved in skin repair and inflammation.