Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spt7 Deletion Reveals Vulnerabilities in <i>Cryptococcus neoformans</i> Stress Adaptation and Virulence.

Microorganisms·2026
Same author

Characterization of PPE19 as a novel mediator of <i>Mycobacterium tuberculosis</i>-macrophage interactions.

mSphere·2025
Same author

Forest citizens and people-centered conservation in the Brazilian Amazon.

Conservation biology : the journal of the Society for Conservation Biology·2025
Same author

Florasulam Is a Potent Inhibitor of <i>Mycobacterium tuberculosis</i> Acetohydroxyacid Synthase and Possesses In Vivo Antituberculosis Activity.

ACS infectious diseases·2025
Same author

Deciphering the functions of Spt20 in the SAGA complex: Implications for Cryptococcus neoformans virulence.

Life sciences·2025
Same author

Whole genome CRISPRi screening identifies druggable vulnerabilities in an isoniazid resistant strain of Mycobacterium tuberculosis.

Nature communications·2024

Related Experiment Video

Updated: Jun 15, 2025

Generation of Fluorescent Protein Fusions in Candida Species
09:27

Generation of Fluorescent Protein Fusions in Candida Species

Published on: March 4, 2017

10.7K

A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus neoformans.

Louis S Phillips-Rose1, Chendi K Yu1, Nicholas P West1

  • 1Australian Infectious Diseases Research Centre, School of Chemistry & Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Journal of Fungi (Basel, Switzerland)
|August 28, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel reporter system for Cryptococcus neoformans, combining fluorescent proteins and counter-selection. This tool enhances the study of this fungal pathogen by improving gene function analysis.

Keywords:
Cryptococcus neoformansamdSfluorescent proteinfungal pathogenfusion genereporter

More Related Videos

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.0K
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

4.2K

Related Experiment Videos

Last Updated: Jun 15, 2025

Generation of Fluorescent Protein Fusions in Candida Species
09:27

Generation of Fluorescent Protein Fusions in Candida Species

Published on: March 4, 2017

10.7K
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.0K
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

4.2K

Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • Genome sequencing has advanced, but understanding gene function remains challenging.
  • Reporter genes are crucial for correlating gene expression with observable phenotypes.
  • Existing reporters like lacZ and fluorescent proteins have limitations in certain organisms.

Purpose of the Study:

  • To create a robust, synthetic, fusion reporter system for Cryptococcus neoformans.
  • To combine versatile fluorophores with the counter-selectable amdS marker.
  • To develop a superior whole-cell assay for interrogating gene function.

Main Methods:

  • Construction of a fusion open reading frame (ORF) reporter system.
  • Generation of multiple reporter variants (composition and orientation).
  • Evaluation of reporter expression using TEF1 and GAL7 promoters.

Main Results:

  • Successful creation of a synthetic fusion reporter system for C. neoformans.
  • Demonstrated differences in expression across reporter variants.
  • Elucidated novel expression tendencies of TEF1 and GAL7 promoters in C. neoformans.

Conclusions:

  • The developed fusion reporter system offers a versatile and effective tool for C. neoformans research.
  • This system provides superior whole-cell assay capabilities compared to traditional methods.
  • The enhanced genetic tools will aid in the manipulation and understanding of C. neoformans.