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An improved method for measuring human tear lysozyme concentration.

P Velos, P M Cherry, D Miller

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |January 1, 1985
    PubMed
    Summary
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    A new, simple method accurately measures human tear lysozyme using radial immunodiffusion. This assay is reliable for diagnosing dry eye disease by quantifying tear lysozyme levels.

    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Immunology

    Background:

    • Existing methods for human tear lysozyme measurement have significant limitations.
    • Accurate quantification of tear lysozyme is crucial for diagnosing and managing ocular surface diseases like dry eye.

    Purpose of the Study:

    • To develop and validate a novel, reliable, and clinically applicable method for measuring human tear lysozyme concentration.
    • To compare tear lysozyme levels in healthy individuals versus those with dry eye syndrome.

    Main Methods:

    • Tear fluid was collected on filter paper discs and eluted in sodium phosphate buffer.
    • Radial immunodiffusion was employed to quantify lysozyme concentration based on precipitation ring diameters.
    • A standard curve was used to determine lysozyme concentration in micrograms per milliliter.

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    Main Results:

    • The mean tear lysozyme concentration in 15 normal subjects was 1.4 ± 0.5 mg/mL.
    • In 10 patients with dry eyes, the mean concentration was significantly lower at 0.7 ± 0.5 mg/mL.
    • The collection, storage, and transport method proved easy to perform and well-tolerated by patients.

    Conclusions:

    • The developed radial immunodiffusion assay is a simple, reliable, and clinically feasible method for measuring tear lysozyme.
    • Reduced tear lysozyme levels may serve as a biomarker for dry eye disease.
    • This technique offers an improvement over previously described, less reliable assays.