An Open-Source 3D-Printed Recording Stage with Customizable Chambers for Ex Vivo Experiments

Area of Science:

• Neuroscience
• Biomedical Engineering
• Physiology

Background:

• Ex vivo experiments are crucial for understanding neurophysiological mechanisms and human physiology.
• Commercially available ex vivo platforms are costly, often unavailable, and lack customization.
• Limitations of current ex vivo systems hinder accessibility and adaptability in research.

Purpose of the Study:

• To design and construct an affordable and adaptable ex vivo recording stage using 3D printing.
• To overcome the financial and functional limitations of existing commercial ex vivo experimental platforms.
• To provide a versatile tool for diverse ex vivo tissue preparations in neuroscience and other fields.

Main Methods:

• Utilized fused deposition modeling (3D printing) to fabricate essential components for an ex vivo laboratory system.
• Developed designs for an accessible, adaptable recording stage with modifiable submersion chambers.
• Focused on a low-cost construction process, with a total cost under $15.00.

Main Results:

• Successfully created a functional and highly adaptable ex vivo recording stage.
• The 3D-printed system offers significant cost savings compared to commercial alternatives.
• The modular design with exchangeable chambers allows for versatile application across various ex vivo tissue types.

Conclusions:

• 3D printing offers a practical solution for creating accessible and customizable ex vivo research tools.
• This low-cost, adaptable system democratizes ex vivo research, particularly in neuroscience.
• The developed platform facilitates novel research by enabling tailored experimental setups for diverse tissue preparations.