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Sperm-origin paternal effects on root stem cell niche differentiation
Tianhe Cheng1, Zhenzhen Liu1, Haiming Li1
1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
Nature
|August 28, 2024
Summary
Paternal genes TREE1 and DAZ3 in Arabidopsis sperm are crucial for normal embryo root development. Genetic deficiencies in these sperm genes cause lasting defects in plant organ differentiation.
Area of Science:
- Plant genetics
- Developmental biology
- Epigenetics
Background:
- Parental genetic contributions to embryogenesis are known, but paternal effects on plant development are poorly understood.
- Both parents contribute to the zygotic transcriptome, suggesting a paternal role in early development.
- Specific paternal effects and molecular pathways remain largely unelucidated.
Purpose of the Study:
- To demonstrate the paternal contribution to early embryogenesis and plant development.
- To investigate the function of TREE1 and DAZ3 in Arabidopsis sperm.
- To elucidate the molecular mechanisms underlying paternal effects on root stem cell niche differentiation.
Main Methods:
- Investigated the expression of TREE1 and DAZ3 in Arabidopsis sperm.
- Generated tree1 daz3 mutant Arabidopsis.
- Analyzed embryo root stem cell niche differentiation and root tip regeneration in mutants.
- Studied the transcriptional regulation of RKD2 by TREE1 and DAZ3.
Main Results:
- TREE1 and DAZ3 are exclusively expressed in Arabidopsis sperm.
- tree1 daz3 mutants exhibited aberrant embryo root stem cell niche differentiation and disrupted root tip regeneration.
- TREE1 and DAZ3 function by suppressing maternal RKD2 transcription.
- These findings reveal enduring paternal effects on plant organ differentiation.
Conclusions:
- Genetic deficiencies in sperm genes (TREE1, DAZ3) can have lasting paternal effects on specific plant organ differentiation.
- Parental-of-origin gene interactions are critical for normal embryogenesis.
- This work introduces a new perspective on how gamete quality impacts plant organ formation.
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